Abstract: This work was done to investigate the biochemical and haematological parameters of aqueous extract of Tithonia diversifolia plant, in mice induced with Plasmodium berghei a plant that is used locally to treat malaria. A total of 25 mice were used in this study and were divided into 5 groups. Group A (control), group B group C, group D and group E were infected with Plasmodium bergei and treated with normal saline, artesunate (50mg/kg), 300mg/kg and 600mg/kg body weight of the extract respectively. MCH – mean cell hemoglobin; MCHC – mean cell hemoglobin concentration; MCV– mean cell volume; PCV – packed cell volume; PLT – platelet count; RBC – red blood cells; WBC – white blood cell count/leukocyte count , N – Neutrophil Count, L-Lymphocyte Count, Hb- Hemoglobin count, M- monocyte count and AST –Aspartate Aminotransferase , ALT- Alanine Aminotransferase and ALP Alkaline phosphatase were evaluated and analysed statistically.