Abstract: Pseudomonas aeruginosa is among the most common pathogen causing nosocomial infections. The objective of this study was to investigate the multidrug resistance pattern of P. aeruginosaisolatesfrom pregnant women and to determine the presence of extended-spectrum-beta-lactamase (ESBLs) genes. A total of 28 isolates of P. aeruginosa were isolated from pregnant women attending antenatal clinic in a tertiary health care Centre in Makurdi, Central Nigeria. Susceptibility to fifteen antimicrobial agents was performed by disk diffusion method. ESBL-genotypic detection of the presence of the genes encoding for bla(TEM) and bla(CTX-M), was studied by a multiplex polymerase chain reaction. The prevalence of ESBLs gene was 10.7% as the presence of genes encoding ESBLs was confirmed in three isolates. P. aeruginosademonstrated the highest resistance rate to clindamycin (96.4%), ceftriaxone (89.3%) and clarithromycin (89.3%). A 100% of the isolates were sensitive to ofloxacin, perfloxacin, ciprofloxacin, gentamycin and streptomycin. Also, all of the isolates were (Multiple antibiotic drug resistance) MDR (resistant to three or more antibiotics). Proper infection control practices and barriers are essential to prevent spreading and outbreaks of ESBL-producing and MDR P. aeruginosa in our environment and consequently in pregnant women.