Abstract: Seven different strains of Trichoderma are isolated from wilt infected leguminous crops of a Madhya Pradesh state and tested for their antagonistic activity against Fusarium (soil borne pathogen) which is expressed as a zone of inhibition in the culture plates. The seven strains are identified as Trichoderma viride, T. harzianum, T. asperellum, T. koningii, T. atroviride, T. longibrachiatum, and T. virens. Upon successful identification, morphological description of the isolated strains. This study aims at selecting the best strain of Trichoderma species (Trichoderma viride) and then preparing a simple bioformulation that is cheap, easy to apply and readily accessible to the farmers. Shelf life of the prepared bioformulation is even checked for 180 days and it is concluded that the number of prop gules start declining from 30th day onwards when the bioformulation is prepared in talc as a carrier material.