Abstract: Background and Aims: Pseudomonas aeruginosa is an opportunistic pathogen. The appearance of the Metallo-β-Lactamases genes and their spread among bacterial pathogens is a matter of concern with regard to the future of antimicrobial therapy.Antimicrobial resistance is an innate feature of bacterial biofilms. Biofilm formation is higher in MDR strains. The present study was undertaken with the aim to find Prevalence of Metallo-β-Lactamases among isolates forming Biofilm, Antibiotic resistance pattern of the isolates and their correlation with biofilm producer. The study was carried out in the Tertiary Care Hospital from the period of February 2013 to August 2013. Total of 60 Multidrug resistant (MDR) Pseudomonas aeruginosa isolated from 638 clinical samples were identified by standard microbiological techniques & the isolates were further tested for Antibiotic susceptibility testing. Results:Of 60 Multidrug resistant (MDR)Pseudomonas aeruginosa, the Metallo-β-Lactamases was seen in 30%& Meropenem resistance was seen in 16.66 %.Highest prevalence of Metallo-β-Lactamases was seen in Pus 41.66% followed by Urine (33.33%) blood (23.07%), sputum (20%), Miscellaneous (30%). Biofilm formation was seen in in 65%. Higher antibiotic resistance was seen in strong biofilm producers as compared to the negative biofilm producers.In our study P. aeruginosa showed (56.67%) resistance to ceftazidime,Cefoperazone(61.67%), netilin (78.33%), ticarcillin (61.67%). P.aeruginosa showed higher sensitivity to Amikacin (83.33%),Meropenem(81.67%),Cefepime (66.67%),tobramycin(80%).