Abstract: A simple, Precise, Accurate method was developed for the estimation of Valganciclovir by RP-HPLC technique. Chromatographic conditions used are stationary phase Ascentis 150mm x 4.6 mm, 5.0, Mobile phase 0.01N potassium dihydrogenphosphate: Methanol in the ratio of 65:35 and flow rate was maintained at 0.8ml/min, detection wave length was 254nm, column temperature was set to 30oC, retention time 2.180min. System suitability parameters were studied by injecting the standard six times and results were well under the acceptance criteria. Linearity study was carried out between 25% to150 % levels, R2 value was found to be as 0.999. Precision was found to be 0.8 for repeatability and 0.4 for intermediate precision.LOD and LOQ are 0.0542µg/ml and 0.1643µg/ml respectively. By using above method assay of marketed formulation was carried out 99.8% was present. Degradation studies of Valganciclovir were done, in all conditions purity threshold was more than purity angle and within the acceptable range. This method can be used for routine analysis of Valganciclovir.