Abstract: The experiment was undertaken in the Farm laboratory of Sher-e-Bangla Agricultural University, Dhaka, during Kharif 1 season, April to July 2013 to examine the response of different levels of nitrogen on morpho-physiology, yield contributing attributes and seed yield of sesame (Sesamum indicum L.) variety BARI Til 4. In this experiment, the treatment consisted of three different N levels viz. N0 = 0 kg N/ha, N1 = 60 kg N/ha and N2 = 120 kg N/ha. The experiment was laid out in a factors Randomized Complete Block Design (RCBD) with three replications. The total treatment were 3 and plot (3x3) 9. Results showed a significant variation among the treatments in respect of the majority of the observed parameters. The N significantly increased morpho-physiological characters - plant height, number of leaves plant-1, fresh and dry weight of shoot and root; yield contributing characters - number of pod plant-1, pod diameter, pod length, seed weight plant-1, seed weight plot-1,thousand seed weight compared to control. The maximum seed yield (1.26 t/ha) was obtained from 60 kg N/ha, whereas (1.01 t/ha) was from 120 kg N/ha which was better than control (0.88 t/ha). Based on the present results, it can be suggested that the use of 60 kg N/ha increased plant morpho-physiological parameters and seed yield of sesame than 120 kg N/ha due to excessive use of this element may produce too much of vegetative growth or toxicity, thus seed production decreased and failed to give highest yield.