Abstract: The effect of aqueous extracts of dried seeds powder of Butea Monosperma plant and Nigella Sativa plant against Ethylene glycol induced renal calculi in albino wistar rats has been studied in this research. A renal calculus was induced in rats by ingesting 0.75% Ethylene glycol in drinking water for one month. Ethylene glycol treated rats showed significant high increase in the level of promoters such as calcium, phosphorous, Potassium, BUN and low concentration of inhibitors such as magnesium and citrate contents in the urine samples. Histopathological studies also confirmed the deposition of calcium oxalate crystals in kidneys. Separate oral administration of Butea Monosperma aqueous suspension to one group of Ethylene glycol induced urolithic rats and Nigella Sativa aqueous suspension to another group of Ethylene glycol induced urolithiatic rats (2g/kg body wt/day upto one month) had reduced the concentration of calcium, oxalate, BUN, Creatinine, Phosphorous, and diminished the crystals deposition in the kidneys. The result of the present study confirmed that Butea Monosperma aqueous extracts and Nigella Sativa aqueous extracts can be used as curative agent for urolithiasis.