Abstract: Abrasion resistance is used to quantify the service life of the textile material in normal use. The aim of this study is to measure the deterioration of three thread fleece knit fabric due to abrasion. To conduct these work three different types of three thread fleece (all cotton, cvc/poly/cotton, all PC) fabrics were selected. The test was carried out in the technical back side of the fabric because of the presence of loose and course fleecy yarn where the possibility of fibre loss is higher than technical face side. The weight loss percentage of the fabric both in grey and finished state were measured at every 500 cycles interval on Martindale instrument according to ISO 12947-3:1998. The result showed that the rate of weight loss percentage was raised with increment of the cycle, and a measurable effect found in the weight loss percentage of polyester based three thread fleece fabric yarn after 500 cycles than the others. It was also observed that for all cotton based fleece fabric variation in loss of weight in grey to finished state was not so remarkable, but the weight loss percentage was found higher in all CVC, polyester, cotton based fleece fabric.