Abstract: Thirty chickpea genotypes were evaluated for moisture stress tolerance imposing by PEG-6000 in growth chamber, at Phytotron facility, Department of Botany, MPKV, Rahuri during Rabi-2013. The germination test and seedling growth under control and moisture stress were recorded to study the genotypic variation for moisture stress tolerance. Ten seeds were sown in germination paper and water stress treatments (0, -0.4 and -0.6 bar solution) create by Polyethylene glycol (PEG-6000). PEG-6000 (Distilled water, 178 and 223 mg) dissolving in 1 L of half strength Hoagland's nutrient solution separately to create water stress treatments of control , -0.4 and -0.6 bar, respectively. The experiment was laid out in a completely randomized design with two replications for each experimental unit. The observations on germination count at every 2 days interval upto 8 days and seedling length and dry matter after 8 days were taken. The genotypic variation was statistically significant for all the parameters. On the basis of stress parameters, the genotypes, NBeG 47-1, PBC-161 and BBG-2 for germination, GJG-1010 and PBC-161 for seedling growth and NBeG 47-1 and PBC-161 for higher dry matter production were found to be moisture stress tolerance.