Abstract: The present study was performed to evaluate the effectiveness of gamma radiation in detoxification of Echis Coloratus snake venom without affecting their immunogenic properties. This was carried out by studying the toxicological, immunological and biological properties of the crude venom. Data revealed that the toxicity of venom after (1.5 and 3 KGy) gamma irradiation was reduced 6.4 and 7.2 times, respectively, compared to the native venom. Immunogenicity was evaluated by performing the double immunodiffusion test; same number of visible lines joined smoothly at the corner was observed with the non-irradiated, as well as, the two dose levels gamma irradiated (1.5 and 3 KGy) snake venom; this result indicating that there was no change in antigenic reactivity.