Abstract: An investigation on quality parameters of honey collected from mustard, litchi and sundarban (multiflora) flora of Bangladesh was undertaken to compare with European Union (EU) quality standard viz., moisture content, electric conductivity, specific rotation, hydroxymethylfurfural (HMF) content, invertase content, antibiotic content and sulfonamides content. Sundarban honey (multifloral) (17.7g/100g) and mustard honey (19.5g/100g) were found in standard moisture condition and the litchi honey was found above the limit of EU standard (20g/100g). All the honey samples were found in the EU standard limit in terms of electrical conductivity and specific rotation. The HMF content was found higher (200.5mg/kg) in sundarban sample and no HMF was observed in the litchi honey. Highest invertase activity (99 IU/kg) was found in the litchi honey where as mustard and sundarban honey showed very low invertase activity with 2.0 IU/kg and 0.0 IU/kg respectively which was far below than EU standard (50 IU/kg). All the samples had shown negative reaction with tetrasensor and sulfasensor. So, it may be concluded that the honey from sundarban was highly overheated. On the other hand, the mustard and litchi honey were overheated and not heated respectively. Sundarban and mustard honey had failed to reach the quality standard to the EU. But litchi honey was found to met the all the EU quality requirements except the moisture content.