Abstract: Objective: We conducted this retrospective descriptive cross sectional study on cholelithiasis diagnosed on abdominal ultrasonography in a tertiary hospital of eastern Nepal. Methodology: Study population consisted of 6278 persons (1381 males & 4897 females) who were examined by abdominal ultrasonography at Koshi Hospital, Biratnagar, Nepal from January 1, 2022 to March 31, 2022. All the patients diagnosed with cholelithiasis were taken from computer record and data was analyzed.