Abstract: Laboratory experiments were performed to evaluate the Pb uptake capacity by three aquatic macrophytes (Scripus mucronatus, Rotala rotundifolia and Myriophyllum intermedium). The selected macrophytes were transferred to the laboratory containing nutrient solution and working Pb standard solutions of different concentrations (1.0, 2.0, 4.0, 8.0 and 16 mg L-1) and harvested at regular time interval of 2, 4, 6, 8 and 10 days. The Pb uptake by these macrophytes showed a linear relationship for S. mucronatus and for R. rotundifolia with the exposure time period (2–10 d). Pb accumulation in the plant parts was higher in the roots for S. mucronatus but reverse in the case of R. rotundifolia and M. intermedium.