Abstract: Transcription factor (TF) binding sites or motifs (TFBMs) are functional cis-regulatory DNA sequences that play an essential role in gene transcriptional regulation. TFs play important roles in cellular physiology, developmental processes and responses to environmental stimuli.The importance of promoter analysis relies on how abundantly TFBSs and TFs are integrated into reference databases. The primary components of promoter are cis acting regulatory regions. Various tools and software’s are being used to analyze and predict regulatory motif within promoter region of a gene. PlantPAN serves as a systematic platform for plant promoter analysis and reconstructs transcriptional regulatory networks. In the present study, we identified various motif including: light responsive elements, cis regulatory elements, core promoter elements and transcription factor binding motifs on the enhancer sequences of tubulin gene participating in variety of functions occurring in cellular processes.