Abstract: The results on the biochemical constituents of rose inflorescence revealed that the levels of lipid, carbohydrate, nitrogen and protein were significantly lower (P<0.05) in infested parts when aphid population were maximum as compared to uninfested ones in most of the cases.The rose plants were mainly infested with two aphid species viz., Macrosiphumrosae (Linnaeus) and Macrosiphumrosaeiformis (Das). The infestation of insect pests caused considerable damage to the individualparts (leaf, stem and inflorescence) of the rose plants and significantly reduced the nutritional constituents at different growth stages. Macrosiphumrosae was found to be the most active pest of rose. These aphids have appeared in the fields especially with the onset of flowering. The loss in biochemical nutritives of plant foliage due to aphid infestation would degrade the quality of the products mainly made from the inflorescence of rose plants. This study reports results obtained from different localities, which may have almost similar ecological and environmental conditions.