Abstract: The aim of this present study was isolation and identification of fungi from rhizosphere soil of papaya (Carica papaya L.) and eggplant (Solanum melongena L.) at BCSIR campus in Rajshahi during the months of 25 Jan-01 Nov2021.The rhizosphere soil were collected at 05 to 15 cm depth of the plants. Several fungi were isolated from the rhizosphere soil of two types of plant by using serial dilution method. Aspergillus flavus, Penicillium notatum, Fusarium oxysporum, Tricoderma harzianum,Penicillium chrysogenum were isolated from the rhizosphere soil of papaya (Carica papaya L.). Fusarium oxysporum,Aspergillus flavus,Aspergillus niger were isolated from the rhizosphere soil of eggplant (Solanum melongena L.).Petri dish containing Potato Dextrose Agar (PDA) medium was used for pure culture until pure fungal colony was found.So Fusarium oxysporum, Aspergillus flavus was the common fungi from the rhizosphere soil of papaya (Carica papaya L.) as well as eggplant (Solanum melongena L.) .Identification of all type of fungi were carried out by culturing on Potato Dextrose Agar (PDA) media and microscopic method.Microorganisms play an vital role in biodegradation of solid agricultural waste as well as help for increasing crop production.