Abstract: I Look out and give an increasing attention has to be focused on understanding bacterial biofilms and this growth relation to human diseases. In this review we explore the biofilm formation, maturation and its detection methods in the context of gram- positive cocci, Staphylococcus aureus. In addition, we discuss diseases and host immune response and current therapies associated with Staph aureus biofilm infections and prevention strategies. Staphylococcus aureus is an important food borne pathogen able to form biofilms.