Abstract: Polycystic ovarian syndrome is most common endocrine disease and metabolic disorder in adolescence and reproductive women. In PCOS women insulin resistance thought to be the uniting pathogenic factor in the development of glucose intolerance, obesity, lipid abnormalities, HTN and coronary artery disease .This study is under taken to measure insulin resistance in PCOS and to see the relationship of insulin resistance with serum lipoprotein ratio. Case control study was done taking 60 women PCOS and 60 age matched healthy women as controls.In all the subjects, concentrations of fasting plasma glucose, serum TG, serum TC and HDL were estimated using enzymatic methods in semiautoanalyser. Fasting serum insulin and was measured by CLIA using Lumax-CLIA microplate reader. HOMA IR, serum LDL concentration, serum TC:HDL ratio, serum TG:HDL ratio and serum LDL:HDL ratio were calculated from estimated parameters.The mean concentrations of all the parameters were significantly increased in women with polycystic ovarian syndrome when compared with healthy women except serum HDL concentration, which was significantly decreased.Insulin resistance was significantly positively correlated with serum lipid profile and serum lipoprotein ratio except with serum HDL, which was significantly negatively correlated.