Abstract: Priming materials are important to enhance germination ability of seeds. One of the materials used was vinegar. This study was conducted to determine effect of vinegar on the germination of eggplant seeds and to identify, which vinegar concentration is suitable for seeds germination. A petri dish method was used in this study with six treatments, replicated three times and arranged Completely Randomized Design (CRD). The treatments composed of five vinegar concentration (10, 1, 0.1, 0.01 and 0.001%) and a control. The parameters includes germination percentage, germination rate and germination index. The result showed that application of 0.001% vinegar concentration showed significant effects in terms of percent germination and germination rate. Application of 10% vinegar concentration has detrimental effect of eggplant seeds. Thus, application of vinegar with 0.001% concentration is effective in germination of eggplant seeds.