Abstract: Soil samples from two different city hospitals were collected pre-treated along with several antibiotics for primary screening of numerous microbes were cultured after serial dilution over sterile nutrient agar plates. A total of three isolated were identified and purified from the samples, further screened for individual antibiotics at their respective varying concentrations and found that isolates 2nd is strong resistant against antibiotics selected in the study. Morphological, biochemical and physiological properties were analysed for all the isolates.