Abstract: This work aims to study the effects of exposure to the vapors of motor gasoline (vehicles fuel), on the antioxidants, trace elements and oxidative stress (MDA), on workers of gasoline station. Results showed that increased duration of the work in the filling station lead to significantly increase in levels of Pb, Hg, Cd and MDA, decreased levels of Se, Cu, Zn, Mg, SOD, CAT, GPx, GRx, GST, ALAD, GSH and TAC. Furthermore, MDA showed positive correlation (P< 0.001) with Pb, Hg, Cd, negative correlation (P<0.001) with SOD, (P<0.01) with GRx and ALAD, (p< 0.05) with Se, Zn, Mg, GSH, CAT and GRx and non-significantly (P> 0.05) negative correlation with Cu and of GST. Level of Pb was negatively correlation with GRx, ALAD (P <0.01), while (p <0.001) with TAC, GSH, GST, GPx and SOD, and (P <0.05) with CAT. Also, Hg gives negative correlation (P< 0.001) with TAC and SOD, (P< 0.05) with CAT, GPx, GRx, ALAD and (P > 0.05) with GSH and GST. Moreover, Cd showed negative correlation with levels of TAC and SOD (p <0.001), (p<0.05) with GSH and ALAD, CAT and GPx, and (P> 0.05) with GRx, GST in workers, compared to healthy control.