Abstract: Polycystic ovarian syndrome is most common endocrine disease and metabolic disorder in adolescence and reproductive women. In PCOS women insulin resistance thought to be the uniting pathogenic factor in the development of glucose intolerance, obesity, lipid abnormalities, HTN and coronary artery disease .This study is under taken to measure insulin resistance in PCOS and to see the relationship of insulin resistance with lipid profile. Case control study was done taking 60 women PCOS and 60 age matched healthy women as controls. In all the subjects, concentrations of fasting plasma glucose, serum TG, serum TC and HDL were estimated using enzymatic methods in semiautoanalyser. Fasting serum insulin and measured by CLIA using Lumax-CLIA microplate reader. HOMA IR, serum LDL concentration, were calculated from estimated parameters. The mean concentrations of all the parameters were significantly increased in women with polycystic ovarian syndrome when compared with healthy women except serum HDL concentration, which was significantly decreased. Insulin resistance was significantly positively correlated with serum lipid profile and except with serum HDL, which was significantly negatively correlated. This study suggests that insulin resistance in PCOS is associated with dyslipidemia. Hence in PCOS women if measurement of insulin resistance and serum lipid profile is done early, the disease progression can be prevented and prevent future complications.