International Journal of Scientific and Research Publications

IJSRP, Volume 3, Issue 2, February 2013 Edition [ISSN 2250-3153]


Salt Tolerant Protease Produced by an Aerobic Species Belonging to the Bacillus Genus Isolated from Saline Soil
      A. G. Jadhav, A. A. Jaybhaye , M. Musaddiq
Abstract: Microbial proteases important hydrolytic enzymes represents largest group of industrial enzymes and have applications in various industries. Proteases capable to in harsh condition of salinity, alkalinity and temperature etc are thus of great concern. Extracellular protease producing bacterium isolated from saline soil of Akola district was related to Bacillus licheniformis on the basis of biochemical and16S rDNA gene sequence similarity. The enzyme was active in the range of pH 6–10 with the optimum at 9.0. Different carbon and nitrogen sources used in the study found increased protease production in Lactose and Yeast extract. The enzyme was highly stable at various NaCl concentrations retained 50% activity after 4hrs. Also, the enzyme was quite stable at higher temperature in the presence of KCl, NaCl and CaCl2. Activity of this was affected by mono and divalent metal ions to varying extent. The study on this enzyme assumes significance with dual extremities of pH and salt also with moderate temperature stability.

Reference this Research Paper (copy & paste below code):

A. G. Jadhav, A. A. Jaybhaye , M. Musaddiq (2018); Salt Tolerant Protease Produced by an Aerobic Species Belonging to the Bacillus Genus Isolated from Saline Soil; Int J Sci Res Publ 3(2) (ISSN: 2250-3153). http://www.ijsrp.org/research-paper-0213.php?rp=P14807
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