IJSRP, Volume 11, Issue 1, January 2021 Edition [ISSN 2250-3153]
M Sharifi and B N kunchirman
Different mangrove soils were screened for bacterial cultures with antimicrobial activity against common pathogens. Out of 80 bacterial isolates obtained from mangrove soils from various regions of India, 20 cultures were found to inhibit most tested pathogenic microbial cultures, however only 3 cultures designated as JF6, MP15 (2) and JF14+1 were found to be non-toxic in sheep blood haemolysis test. Anti-microbial activity of these cultures was found to be extracellular and maximum activity was observed when grown at pH 6 at 30℃ and after 36 hr of incubation. The activity of the culture was stable even after treatment with proteinase K and temperature up to 80℃. By biochemical and molecular identification methods, these cultures were identified as Bacillus tequilensis.