Abstract: In this research work, one of Myanmar indigenous medicinal plants, Chamaecyparis pisifera (Siebold & Zucc.) Endl., Myanmar name Kyauk pan was selected for chemical analysis. It was collected from Forest Research Institute (FRI) campus, Yezin, Zaeyar Thiri Township, Naypyitaw Region, Myanmar. Moreover, a potent anti-inflammatory activity of the root of C. pisifera was determined by carrageenan induced inflammation method. This plant showed remarkable inhibitory activity in high dose and also the activity followed by the remaining medium and low dose. High dose was found to possess significant anti-inflammatory activity, which supported the traditional application of the test plant in acute inflammatory activity.