Abstract: A reference value for glycosylated haemoglobin in the Kenyan population in Kiambu County was developed. 600 normal adult individuals were recruited in the study, 7 (1.2%) of their sample were not analysed as they were positive for HIV (5) and HbsAg (2). The remaining 593 study participants had representation of male 325 (54.8%) and female 268 (45.2%).Six millitres of blood was obtained from the participants three millitres put in EDTA vacuitainer for analysis of glycosylated haemoglobin and three millilitres put in a plain vacuitainer for screening of HIV, HbsAg and VDRL.Statistical package for the social sciences (SPSS) programme (version 21) was used for data analysis.