Abstract: Incidents of fire within hospitals can lead to a multitude of injuries, significant property damage, and even loss of life. Understanding and following fire safety measures and readiness among health workers can significantly protect lives. The aim of the study was to assess health workers knowledge and readiness for fire safety in Lusaka, Zambia. A cross-sectional survey was conducted among 52 healthcare workers in Lusaka, Zambia at two public hospitals, the University Teaching Hospital and Sikanze Police Hospital. SPSS IBM statistics were used to analyze the data in the form of percentages and proportions. The bulk of the 52 respondents that took part in this study were females (55.8%), as opposed to males (44.2%). Most health workers in the survey (50.0%) were between the ages of 38 and 47, and the majority (30.8%) had worked at the hospital for 1 to 5 years. The study also showed that 49 (94.2%) of the health workers in the sample had graduated from tertiary education. Most respondents had sufficient awareness of fire safety, according to the research but lack the information and training necessary to apply fire safety preparedness techniques. Thus, the study suggests that staff should receive training on how to operate fire apparatus and participate in emergency evacuation exercises or simulations.