Abstract: Introduction: The prevalence of gallstones varies between the population in each country and the ethnicity of the country. Incidence is reported in 11-36% of the world s population, 10% in the population of Western countries. In developing countries the prevalence varies from 15-25%. Research on the analysis of the bile acid composition of the microbiota shows the role of bacteria in the formation of pigment stones. From several patients after cholecystectomy, infection and sepsis occurred, the patient s bile culture - bacteria were found. Methods: To analyze the type of gallstones macroscopically with culture to see the presence or absence of infection with microorganisms in bile. patients with cholelithiasis or gallstones at HAM Hospital and Network Hospital.