Abstract: Nile tilapia (Oreochromis niloticus) is one of the important economic value fishery commodities and has been cultivated commercially. The main problem experienced is the low seed production through hatchery units due to the low survival rate, especially in the larval stage. This research was conducted at CV. Aquaculture Lestari, Makassar aims to evaluate the effect of vitamin B complex on the survival, growth, and stress resistance of nile tilapia (O. niloticus) larvae. The study used a container in the form of a black plastic basin with a capacity of 40 L which would be filled with 30 L of media water. The test animals used were 3-day old nile tilapia larvae which were kept for 30 days. The feed used is artificial feed in the form of powder and pellets. The study was designed using a completely randomized design consisting of 4 doses of vitamin B complex treatment with 3 replications each.