Abstract: Prey densities were estimated in Satkosia Tiger Reserve, Odisha, India from January 2015 to December 2015 by using line transect distance methods. Season wise availability of prey density was collected. The pre-monsoon and Post-monsoon seasons prey density data was analyzed separately. In total, five prey species were recorded on the transect lines studied both in the core and buffer area of the reserve having an area of 964 km2.