IJSRP, Volume 7, Issue 11, November 2017 Edition [ISSN 2250-3153]
K.MaryManjushaRani, Dr. Raj kumar
Recombinant vectors are valuable tools in the biopharmaceutical industry with a number of novel vectors being emerged every day. The aim of this project is to design a novel double gene bacterial expression vector where the two genes can be controlled individually. The double gene expression vector contains two independent transcriptional units. (a) The first transcriptional unit comprises sequences for the osmotic regulated promoter, restriction site for insertion of polypeptide and a transcription termination and (b) The second transcriptional unit comprises sequences for the T7 promoter, MCS for the insertion of polypeptide and a transcriptional termination sequence. The double gene expression vector (pUB-S-X-T7) was constructed by ligating the ClaI and PvuII fragment (4000bp) form the pUB-S-X plasmid and ClaI and ScaI fragment (1000bp) from pUB-T7 plasmid. The presence of two independent transcriptional units was confirmed by colony PCR.