Abstract: The aim of this Study is to design and implement Geographic Information System (GIS) based spatial analysis and hotspot detection of Kidney disease of Wilgamuwa Division, MataleDistrict. The Development of this model is motivated to provide opportunity for healthcare practitioner to gain access to information that can aid him/her to locate the areas of patients and to take necessary measures and to implement awareness programs to reduce the number of kidney disease patients. Although considerable research has been for health GIS applications, two challenges still need to be addressed, this relate to health mapping methods and lack of accessibility to an organized data model. To handle this problem we design a Database GIS for hospital staff to support data sharing and representation. The developed model makes it possible for healthcare practitioner to locate the patients. The study explores the use of open source software, ARCGIS 10.1. The analyzed data and the hotspot analysis will pave the way to the medical practitioners to locate the high risk and low risk areas and to identify the relationship of the distribution of patients with the geographical conditions.