Abstract: Wheat is one of the most widely grown food grain crop in the world. Approximately five percent of the worldwide wheat production is comprised of durum wheat. Durum wheat (Triticum durum Desf.) is mainly produced and consumed in the Mediterranean region, it is used to produce several specific endproducts. Diversity analysis is important for deciphering genetic relationship including parentage and for the efficient management of germplasm and thereby, use in breeding of improved varieties.