Abstract: The analysis of antifungal activity of aqueous- ethanolic extract of leaves of Calotropis procera R.Br. against Botrytis cinerea, Rhizopus oryzae, Fusarium oxysporum was carried out in disc method. The zone of inhibition produced by the crude 50% aqueous ethanolic leaf extract against sensitive fungi was examined. The results obtained showed that 50% aqueous ethanolic leaf extract of Calotropis procera, has inhibitory effect on the growth of isolates. The effect exhibited by ethanolic extract of leaves was significant. 50% aqueous- ethanolic extract of leaves of Calotropis procera R.Br. was partitioned sequentially in petroleum ether, benzene and chloroform and tested for antifungal activity. It was observed that sample obtained from chloroform portion showed most inhibitory effect. Samples obtained from petroleum ether and benzene portion also showed significant inhibitory effect on the growth of isolates.