Abstract: It has become increasingly obvious that continued reliance on fossil fuel energy resources is unsustainable, owing to both depleting world reserves and the green house gas emissions associated with their use. Therefore, there are vigorous research initiatives aimed at developing alternative renewable and potentially carbon neutral Biofuels as alternative energy resources. Algae as a feedstock is emerging at the forefront of biofuel research due to increasing awareness of global energy issues in conjunction with the production limitations of agriculture-based oilseed crops. The present paper is focused on experimentally investigating the influence of injection pressure and injection timing on brake specific fuel consumption (BSFC), brake thermal efficiency (BTE), unburned hydrocarbon (UBHC), carbon monoxide (CO), oxides of nitrogen (NOx) and smoke opacity of a single cylinder compression ignition engine when blends (20%) of algal oil methyl ester are used as a fuel. Engine performance test show that algae oil as a fuel does not differ greatly from that of diesel. The results showed a better performance and reduced emissions at an injection pressure of 200bar..