Abstract: In vitro evaluation of Bioagents against both Fusarium oxysporum and Pleurotus sajor-caju revealed that all the bioagents more or less suppress the growth of Fusarium oxysporum. Out of six bioagents, Bacillus subtilis-115 expressed the efficient antagonistic activity against Fusarium oxysporum followed by P. flourescens-105, P. flourescens- 104 and P. flourescens-103. Among these bioagents tested P. flourescens- 103 revealed more inhibitory effect against pathogen but also inhibited the mycelial growth of Pleurotus sajor-caju strongly. The bioagents Bacillus subtilis-115, P. flourescens-105 and P. flourescens-104 revealed less antagonistic activity against P. sajor-caju but their inhibitory effect against the pathogen was very strong.