Abstract: The kinetic study, cover all the important aspects of chemical reaction including mechanism. The outcome of investigations indirectly explain the path of certain complex biochemical processes. The role of the organic phosphate ester in chemistry and particularly in biochemistry were recognized quite early because of common C—N—P linkages present in nucleotides. With this view, investigations of the kinetic study of the hydrolysis of 2-ethyl, 6-methylaniline phosphate ester has been made in a wide range of various experimental conditions at pH 1.24. The rate of coefficents of temperature effect have been determined. The reactive species has been found to undergo hydrolysis with common P—N bond fission, with bimolecular reaction path, since the entropy of a activation energy falls in the range.