IJSRP, Volume 4, Issue 10, October 2014 Edition [ISSN 2250-3153]
Fakhreldin A. Hussien, Magda A. Osman, Tagelsir I.M. Idris
This study was designed to examine the effects of liquid media supports, gelling agents and liquid overlays on growth and multiplication of ginger. The use of luffa coir as physical support for explants in liquid media enhanced growth compared to other types of supports. Guar gum revealed a promising gelling potential as an alternative for agar. In concentration of 30 g/l, guar as a sole gelling agent, increased shoot and leaf numbers significantly. Enhanced multiplication was obtained upon supply of a 10 ml liquid overlay aliquot per vessel composed of MS salts plus 4 mg/l BA onto the solid multiplication medium. The use of luffa coir and guar gum propose effective alternatives to the high cost agar and may help in reducing cost of tissue culture protocols especially at commercial level.