Abstract: Protein containing WD-repeats participate in a wide range of cellular functions, they are generally involved in regulatory mechanisms concerning chromatin assembly, cell cycle control, signal transduction, RNA processing, apoptosis and vesicular trafficking. lethal(2)denticleless is a recently identified novel class of Protein containing WD-repeats. Bioinformatic analysis has shown conservation of critical functional motifs among the human L2DTL, mouse L2dtl and Drosophila l(2)dtl proteins. The function of l(2)dtl is still under speculation but the studies suggest that L2DTL and PCNA are involved in CDT1 degradation after DNA damage and cell cycle regulation. The gene expression studies have shown that l(2)dtl levels are altered in different condition and even found up-regulated in under galactose challenge conditions in human. l(2)dtl was chosen for its potential regulation by InsP3R mediated intracellular Ca2+ signaling. This study indicates a possible role of l(2)dtl in InsP3R mediated intracellular Ca2+ signaling.