Abstract: Forty-five Jersey Sahiwal cows selected from nearby villages from tirupati. Jersey Sahiwal were divided into three equal groups i.e. cyclic, non cyclic and endometritic. Blood samples were collected from all the experimental animals, serum was separated and stored at -20°C until assayed for glucose (g/dl), total protein (g/dl), urea (mg/dl) and cholesterol (mg/dl). The results revealed significantly (P<0.05) higher values of glucose (58.08 ± 2.59) and cholesterol (290.72 ± 15.95) in endometritic cows as compared to cyclic (50.72 ± 1.12, 199.12 ± 9.38) and non-cyclic cows (50.56 ± 1.12, 202.96 ± 14.84). Total protein level differed significantly (P<0.05) among cows of all the three groups, being highest in endometritic (19.16 ± 1.00), followed by non cyclic (15.23 ± 0.89) and lowest in cyclic (9.19 ± 0.45) cows. However, serum level of urea did not differ in cyclic (30.88 ± 2.42), non cyclic (33.80 ± 3.43) and endometritic (37.12 ± 3.45) animals, although highest value was recorded in endometritic animals.