Abstract: Bats were captured by using nets and hand picking and then chromosome preparations were made from bone marrow cells according to Lee and Elder’s (1980) method cited in Hillis et al. (1996) with some modifications. Somatic metaphase chromosomes of bat specimens collected from Arbaminch, Koka, Merehabete and Ziway regions in Ethiopia were studied. The specimens were identified belong to the following families and species. Pipistrellus pipistrellus and Scotophilus dinganii or S. viridis (family Vespertilionidae), Micropteropus pusillus (family Pteropidae), Chaerephone pumila (family Molossidae) and a taxonomically yet unidentified specimen collected from Merehabete region.