Abstract: Some naturally occurring antagonistic microorganisms has performed great potential to antagonize plant pathogens, hence, biocontrol involving the use of such beneficial microorganisms for plant protection is being considered as a viable substitute to reduce the use of chemical pesticides [1,2]. These microorganisms need to be multiplied and kept viable often for long period under storage. To ensure this purpose some biodegradable wastes i.e., Neem cake, Jatropha cake, Mahua cake and Karanja cake were evaluated under current study. Neemcake remains best substrate in supporting population of Trichoderma harzianum under laboratory. The conidial form of T. harzianum could be stored about 4 months when cakes were added with one of the carbon source; Lactose. An increase in number of viable propagules (CFUs/g) was measured from all the substrates when at 15 DAI, CFUs increasesto 42 from 30.67(plane Neem cake) and at 30 DAI, 64.67 from 42 (plane Neem cake) in lactose mixed Neem cake.