Abstract: This study focuses on to determine the optimization of bio ethanol plant in Kenana to produce product at high quality and maximum income. The different samples of (Beer, mash ,molasses ,and ethanol at different concentration (75% , 92% ,99.8%) for different unit was taken and analyzed and studies their result to determined the properties which effect in the quality of ethanol and production rat like -Total reducing sugar (TRS), polarization(pol), Brix, conductivity, Acidity, appearance, ethanol concentration, (pH) , yeast concentration , density .