Abstract: TrinuclearCu(II) complex was designed and synthesized. The binuclear Cu(II) complex was prepared and then condensed with a mononuclear Cu(II) complex to form trinuclear complex as shown below. All the complexes were characterized by using FT-IR and UV-Visible spectral studies. ESR spectra and magnetic moments of the trinuclearCu(II) complex show the presence of antiferromagnetic coupling.The electrochemical properties of these complexes were studied in the range of 0 V to -1.4 V. The mononuclear Cu(II) complex shows single electron reduction potential at (Epc= - 1.22V), the binuclear Cu(II) complex shows two quasireversible single electron reduction potentials at (E1pc= - 0.76 V and E2pc = -1.23 V) whereas, the trinuclear Cu(II) complex shows three quasireversible single electron reduction potentials at (E1pc = -0.67 V , E2pc= -0.935 V and E3pc= -1.17 V). The binding interaction of the complexes with calf thymus-DNA was studied using absorption and fluorescence spectral techniques.