Abstract: The efficiency of Parthenium hysterophorus L., as potential feed stock was investigated by using different pretreatment methods. P.hysterophorus L., being declared invasive weed, is an obnoxious flowering plant which is a big challenge to all attempts of control. Although several eradication measures have been undertaken in this regard for many years, not a single method is yet an option for the total eradication of Parthenium.Thus, the status of Parthenium management is visualized with respect to large-scale utilization. Therefore, in the present work, Parthenium is chosen to comparatively analyze its efficiency as a potential feed stock. Crop biomass mainly consists of cellulose, hemicelluloses and lignin, which act as limiting factor for being used as a potential feed stock. Thus, conversion of hemicelluloses present in the lignocellulosic biomass into xylose is one of the major steps in preparing the Parthenium as a potential feed stock.