Abstract: Bacopa monnieri L. (family Scrophulariaceae) is a traditional medicinal plant in India, commonly known as “brahmi”. Used for different diseases such as- nervous disorder, respiratory problem, leprosy, splenomegaly, skin disease etc. In the present study, the ethanolic and aqueous extract of the leaf callus of Bacopa monnieri was investigated for its phytochemical analysis by modified Kirby-Bauer diffusion method.The finding of this study revealedthat the extract of the leaf callus of Bacopa monnieri revealed the presence of Tannins,flavonoids, glycosides, terpenoids, saponins, and steroids and absence of Anthroquione,Phenolic etc.