Abstract: Background: Calcium oxalate test is currently the chemical method employed in testing endpoint of decalcification in acid decalcification. However, there are challenges with this existing method used; firstly, the method takes a long duration of 15-30 minutes to complete; secondly, the method uses ammonia which is a highly irritating gas with a sharp suffocating odor necessitating its use in a fume hood; thirdly, exposure to ammonia gas through breathing, swallowing or skin contact causes burning and irritation of the contact surfaces, coughing and can result in blindness, lung damage or death; fourthly, ammonia loses potency over time and lastly, containers of ammonia may explode when exposed to high heat. Additionally, when a high concentration of ammonia is mixed with air, it can explode when ignited. Accordingly, there is a need to device a new chemical method for testing endpoint of decalcification in acid decalcification.