Abstract: Cervical cancer remains a public health concern, despite availability of National policy guidelines in early detection of the disease, and prompt initiation on treatment. Screening through Pap smear test or visual inspection are the primary methods recommended for use by Ministry of Health. In Kisumu County the high HIV prevalence, coupled with cervical cancer disease threatens better female population outcomes in the region. Currently, the uptake of cervical cancer screening in female health workers in Kisumu County remains unknown. This study set out to determine the factors associated with uptake of cervical cancer screening services among female health workers in level 4 health facilities within Kisumu County.