IJSRP, Volume 9, Issue 8, August 2019 Edition [ISSN 2250-3153]
Binayanti, Hilal Anshary, Andi Parenrengi, A. Citra Malina
Increased production of lithopanaeus vannamei vannamei shrimp continues to be pursued, one of which is by increasing shrimp immune response to infection with WSSV disease. This study aims to evaluate the best dosage of dsRNA VP19 WSSV in-vitro production to increase the immune response of vannamei shrimp (L. vannamei). The isolation of the VP19 gene and the construction of the VP19 vaccines were carried out using the Megascript kit with VP 19 genomic DNA as a template. Vaccination was carried out by injection method on vannamei shrimp with an average weight of 10.5 ± 3.5 g and a length of 11.05 ± 1.95 cm.