Abstract: Vitamin D receptor (VDR) gene is recognized as candidate gene for susceptibility to Type 2 diabetes mellitus (T2DM). The aim of this study was to investigate the association between VDR gene polymorphisms and T2DM in Moroccans patients. Materials and Methods. 176 clinically diagnosed T2DM patients and 177 healthy controls from the Moroccans population were recruited. BsmI(rs1544410), FokI(rs10735810) and ApaI (rs7975232) single nucleotide polymorphisms (SNPS) of the VDR gene were determined using polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP). A Vitamin D level was determined using ELISA. Results. The prevalence of Vitamin D deficiency and insufficiency is significantly higher in patients with T2DM than in the control subjects.